Oligos

NGS DNA Base Phosphorothioate Link HPLC purification - 10 nmol

4.06
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  • Cat.Number : BA-NGSHP-PT-001
  • Availability :
    In production

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Next-Generation Sequencing (NGS) is a high-throughput technology allowing the massive sequencing of nucleic acids following a DNA library preparation.

The high demand for low cost sequencing has given rise to a number of high-throughput sequencing technologies. These efforts have been funded by public and private institutions as well as privately researched and commercialized by biotechnology companies. High-throughput sequencing technologies are intended to lower the cost of sequencing DNA libraries beyond what is possible with the current dye-terminator method based on DNA separation by capillary electrophoresis. Many of the new high-throughput technologies use methods that parallelize the sequencing process, producing thousands or millions of sequences at once.

Eurogentec can support your sequencing projects by offering dedicated NGS grade oligonucleotides. Specially produced in order to avoid cross contamination they reduce barcode misalignment during multiplex next generation sequencing projects. Discover also our high quality Universal Primers. These predefined and easy to order catalog oligonucleotides are perfectly suited for sequencing projects after PCR amplification.

Quality: Low cross contamination (<0,1%)
Length: from 20 to 85 bases
Quantity: 10 nmol minimum delivered
Purification: HPLC or Cartridge
QC: 100% QC checked by Maldi-TOF MS
5’ Modifications: 5’ Phosphate / 5’ Biotin-TEG
Bases Option: Phosphorothioate bond
Wobble Bases: Available at no additional cost
Format: dried in tubes
Free shipping

Specifications

Chemistry
Linkage
  • Phosphorothioate link
Molecular Mass/ Weight
  • -
Modification
Modification Position
  • 3'
  • 5'
  • IN
Quantity & Purity
Purification Method
  • RP-HPLC
Storage & stability
Form
  • Dried
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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