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PCR & qPCR

Reverse Transcriptase Core Kit

€543.00
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  • Cat.Number : RT-RTCK-03
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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The universal Reverse Transcriptase Core kit can be combined with each qPCR Core kit or qPCR MasterMix available from Eurogentec. As the RT buffer and the PCR buffer are harmonized, optimal results will be obtained with this combination. The Reverse Transcriptase Core kit contains enough reagents for up to 300 - 10 µl reactions, using the EuroScript RT enzyme and the RNase Inhibitor. The kit contains a 10x buffer, dNTP mix, EuroScript, RNase Inhibitor and 25 mM MgCl2, oligo d(T) primers, random nonamers and RNase free water tubes.

Specifications

Packaging
Kits components
  • Each Kit of 300 reactions contains: - One bottle (1.4 ml) of 10x reaction buffer (black cap) containing KCl and Tris-HCl - One tube (75 µl) of EuroScript reverse transcriptase (white cap) containing Moloney Murine leukemia virus reverse transcriptase, 6250 U at 50 U/µl - One tube (120 µl) of RNase Inhibitor (purple cap) containing RNase inhibitor 4000U at 20 U/µl - One tube (1.25 ml) of 2.5 mM dNTP Mix (green cap) containing dATP, dCTP, dGTP and dTTP in autoclaved, deionized water titrated with NaOH to pH 7.0 - One tube (1.5 ml) of 25 mM MgCl2 (orange cap) - One tube (150 µl) of Oligo d(T)15VN (yellow cap) containing 50 µM oligodeoxynucleotides of sequence d(T)15VN in 10 nM Tris-HCL, pH 8.3 - One tube (150 µl ) of Random nonamers (pink cap) containing 50 µM short oligonucleotides of random sequence (d(N)9) in 10 mM Tris-HCL pH 8.3 - One tube (1.75 mL) of RNase free water (plain cap) containing DEPC water. Each kit of 500 reactions contains: - One bottle (2.5 ml) of 10x reaction buffer (black cap) containing KCl and Tris-HCl - One tube (125 µl) of EuroScript reverse transcriptase (white cap) containing Moloney Murine leukemia virus reverse transcriptase, 6250 U at 50 U/µl - One tube (200 µl) of RNase Inhibitor (purple cap) containing RNase inhibitor 4000U at 20 U/µl - One tube (1.25 ml) of 2.5 mM dNTP Mix (green cap) containing dATP, dCTP, dGTP and dTTP in autoclaved, deionized water titrated with NaOH to pH 7.0 - One tube (1.5 ml) of 25 mM MgCl2 (orange cap) - One tube (250 µl) of Oligo d(T)15VN (yellow cap) containing 50 µM oligodeoxynucleotides of sequence d(T)15VN in 10 nM Tris-HCL, pH 8.3 - One tube (250 µl ) of Random nonamers (pink cap) containing 50 µM short oligonucleotides of random sequence (d(N)9) in 10 mM Tris-HCL pH 8.3 - One tube (1.75 mL) of RNase free water (plain cap) containing DEPC water.
Properties
Format
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Reverse Transcriptase Core kit should be stored at -65 °C to -75 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year. For short term storage the Reverse Transcriptase Core kit can be stored at -15 °C to -25 °C for up to 6 months.
Activity
Template type
  • RNA
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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