PCR & qPCR

Red'y'GoldStar® PCR Mix

112.00
Check your price
  • Cat.Number : PK-0064-02R
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Size

Alternative choices

Quantity

The Red'y'Gold Mix Mix kit contains all PCR reaction componants except the primers and the template. It is a ready-to-use formulation at 2x concentration. The kit contains sufficient reagents for 200 PCR reactions of 50 µl and an additional tube of MgCl2 to adjust the concentration if required.
Sample contains enough reagents for 40 reactions.

An easy-to-use solution:
- 2x MasterMix containing Taq DNA polymerase, dNTPs, MgCl2, buffer and red loading dye
- Extra tube of 25 mM MgCl2
- Just add primers and DNA

Improved assay reproducibility:
By handling watery solutions instead of pipetting 50 % glycerol Taq DNA polymerase solution, pipetting mistakes will be avoided and more reproducible results will be achieved.

Reduced risks
Minimum risk of contamination by minimizing pipetting steps.

Quality Control Each lot is tested for activity in PCR and a specific PCR product will be amplified after 35 cycles. 10 % of the PCR products are visualized on a 1.5 % agarose gel. The PCR is performed in duplex, using serial dilutions of 100 ng, 10 ng, 0.1 ng DNA per 50 µl PCR.

Specifications

Properties
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • Red’y’Gold Mix can be stored at -20 °C (in a constant temperature freezer) for 24 months or at 4 °C for 3months. Do not repeat more than 10 freeze/thaw cycles.
Activity
Template type
  • DNA
Unit definition
  • One unit of enzyme is defined as the amount required for incorporation of 10 nmoles of dNTPs into acid - insoluble material after 30 minutes amplification at 72 °C under the standard reaction conditions.
Usage
  • Research use
Codes
Code Nacres
  • NA.55

You may also be interested in the following product(s)

IMG-EN-NU-0010-10-02.jpg

dNTP Mix 20 µmoles

Cat.Number : NU-0010-10
51.00 Excl. Tax
Picture showing a Mupid One

Mupid® One Electrophoresis System Complete Apparatus

Cat.Number : MU-0041-
638.00 Excl. Tax
Tube of SmartLadder

SmartLadder - 200 to 10000 bp

Cat.Number : MW-1700-10
270.00 Excl. Tax