Custom Peptide Synthesis
Fluorescent Dye-Labeled Peptides
We are pleased to offer a broad range of premium fluorescent dyes that span the whole visible and near infrared spectrum, for incorporation into your custom peptides.
Our Offer
A peptide can be labeled with a single or multiple dye(s) and/or quencher(s) at the peptide N-terminus, C-terminus, or internally, for applications in protein interaction studies, to trace the presence of biomolecules in cells, enzyme activity assays, etc.
Fig: Uptake of CPP by HeLa cells. HeLa cells were incubated with OptiMEM medium containing 10 uM FITC-LC-Antennapedia, cat# AS-24175, AS-24176 for 1 h, washed and analyzed by fluorescence microscopy.
Our fluorescent dyes span the whole visible and near infrared spectrum
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HiLyte™ Fluor Dyes (pH insensitive)
HiLyte™ Fluor dyes are ideal for applications at high or low pH (<4 or >11). These dyes also exhibit higher sensitivities, owing to their enhanced intensity and photostability.
| Name | Abs/Em | Applications | |
|---|---|---|---|
| HiLyte™ Fluor 488 | • | 497/525 nm | FRET Assays, tracing or interaction studies |
| HiLyte™ Fluor 555 | • | 550/566 nm | FRET, Labeling |
| HiLyte™ Fluor 647 | • | 650/675 nm | FRET/TR-FRET, Labeling |
| HiLyte™ Fluor 750 | • | 753/778 nm | Imaging |
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Classical Dyes
Classical dyes such as FAM, TAMRA and the CyLyte Fluor dyes (Cy® Dyes analogues) are a great cost-effective choice when operational pH range of your application is flexible.
| Name | Abs/Em | Applications | |
|---|---|---|---|
| Mca | • | 325/393 nm | FRET |
| AMC | • | 351/430 nm | Fluorogenic substrates |
| AFC | • | 382/480 nm | Fluorogenic substrates |
| EDANS | • | 335/493 nm | FRET |
| FAM | • | 492/518 nm | FRET, Labeling |
| FITC | • | 494/519 nm | Labeling |
| Rh110 | • | 501/527 nm | Fluorogenic substrates |
| CyLyte Fluor 3 | • | 550/564 nm | FRET, Labeling |
| TAMRA | • | 541/568 nm | FRET, Labeling |
| Rox | • | 568/591 nm | FRET, Labeling |
| CyLyte Fluor 5 | • | 648/663 nm | FRET/TR-FRET, Labeling |
| CyLyte Fluor 7 | • | 750/773 nm | FRET, Labeling |
Featured Citations
pH-and Temperature-Dependent Peptide Binding to the Lactococcus lactis Oligopeptide-Binding Protein A Measured with a Fluorescence Anisotropy Assay.
Stevie Norcross, Ashwin Sunderraj, Mathew Tantama
ACS Omega 2019, 4, 2812-2822, Feb 2006 2019 DOI: 10.1021/acsomega.8b02427From AnaSpec (Eurogentec's US subsidiary): The sulforhodamine 101 labeled bradykinin peptides (SR101-RPPGFSPFR and RPPGFSPFRK-SR101) were synthesized and HPLC-purified (>90% purity)
Redox Kinetics of the Amyloid-β-Cu Complex and Its Biological Implications.
Paul Girvan, Xiangyu Teng, Nicholas J. Brooks, Geoffrey S. Baldwin, and Liming Ying
Biochemistry 2018, 57, 6228-6233 DOI : 10.1021/acs.biochem.8b00133From AnaSpec (Eurogentec's US subsidiary): Truncated fluorescently labeled amyloid-beta peptide containing metal binding residues to study its metal interaction characteristics and kinetics.
Isolating and Expanding Endothelial Progenitor Cells from Peripheral Blood on Peptide‐functionalized Polystyrene Surfaces.
Mohamed A. Elkhodiry, Mariève D. Boulanger, Omar Bashth, Jean‐François Tanguay, Gaétan Laroche, Corinne A. Hoesli
Biotechnology and Bioengineering 2019, 116, 10, 2598-2609 DOI: 10.1002/bit.27107From AnaSpec (Eurogentec's US subsidiary): The RGD-containing fluorophore tagged peptide, referred to as RGD-TAMRA was synthesized and HPLC-purified (>90% purity)
Related products & services
Custom Peptides Synthesis
From simple to complex sequences
FRET peptides
We offer a full-range of dye-quencher FRET pairs
TR-FRET peptides
We produce custom peptides labeled with lanthanide metal as donor
Labeling Reagents
Get access to a large collection of molecules
Custom peptide synthesis
We deliver fluorescent dye-labeled peptides in research grade or as critical raw material.