Our dye/quencher labeling offer

We offer a full-range of dye-quencher FRET pairs and fluorogenic substrates.
The tables below list the dyes and quenchers available to label your custom peptides

FRET Pairs
Dye (donor) Quencher (acceptor) Donor Ex/Em
Mca Dnp
•

 325/393 nm
EDANS DABCYL, DABCYL Plus, QXL®490
•

 335/493 nm
FAM QXL®520
•

 492/518 nm
FITC QXL®520
•

 494/519 nm
HiLyte™ Fluor 488 QXL®520
•

 502/527 nm
CyLyte Fluor 3 QXL®570
•

 550/564 nm
HiLyte™ Fluor 555 QXL®570
•

 550/566 nm
TAMRA QXL®570
•

 541/568 nm
Rox QXL®610
•

 568/591 nm
CyLyte Fluor 5 QXL®670
•

 648/663 nm
HiLyte™ Fluor 647 QXL®670
•

 650/675 nm
CyLyte Fluor 7 IR-QXL®
•

 750/773 nm

QXL® and Dye pairings are available from our custom labeling services & from our catalog SensoLyte® protease activity assay kits
(ie., QXL® 520 - HiLyte™ Fluor 488, QXL® 520 - FAM)

Fluorogenic Substrates

Fluorogenic substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released).

Fluorogenic substrate dyes
Dye Ex/Em
AMC
•

 351/430 nm
AFC
•

 382/480 nm
Rh110
•

 501/527 nm

Why use FRET?

FRETand fluorogenic substrates are more sensitive than chromogenic substrates with linear dynamic range and great reproducibility.
FRET-based peptides are used in a variety of applications, with high benefit when used as protease substrates in high-throughput inhibitor screening & drug discovery.

Customize your FRET Peptide

Benefits

Using Dyes and Quenchers from AnaSpec (Eurogentec's US subsidiary) offers many advantages:

  • Unique collection of proprietary quenchers (QXL®) which tandem with the most popular dyes
  • Top quality HiLyte™ Fluor dyes
  • Access to the Cy dyes at an affordable price (CyLyte dyes which have identical structures to the trademarked Cy®)
  • Longer wavelengths for improved sensitivities
  • Easy adaptation to high throughput screening assays

Applications

  • Protease activity detection & measurement
  • Protease-based drug target screening and discovery
  • High throughput screening of protease inhibitors

Featured Citations

FRET-based trilateration of probes bound within functional ryanodine receptors.

Bengt Svensson,Tetsuro Oda, Florentin R.Nitu, Yi Yang, Iustin Cornea, Ye Chen-Izu, James D.Fessenden, Donald M.Bers, David D.Thomas, Razvan L.Cornea1

Biophysical journal. 2014 Nov 4;107(9):2037-48. https://doi.org/10.1016/j.bpj.2014.09.029

From AnaSpec (Eurogentec's US subsidiary) : DPc10 peptides either unlabeled, or labeled with HiLyte Fluor 647 (HF647)

Selective Homogeneous Assay for Circulating Endopeptidase Fibroblast Activation Protein (FAP)

Travis W. Bainbridge, Diana Ronai Dunshee, Noelyn M. Kljavin, Nicholas J. Skelton, Junichiro Sonoda and James A. Ernst

Nature Scientific Report 2017 Oct 2;7 : 12524. 10.1038/s41598-017-12900-8

From AnaSpec (Eurogentec's US subsidiary) : Fret peptides containing an amine-terminal donor (HyLite Fluor 488 or Cy5.5 for aP NIRF) and a dark quencher (QXL 520 or QSY21 for aP NIRF) conjugated to a supplementary C-terminal lysine

AMCA to TAMRA long range resonance energy transfer on a flexible peptide.

A. Synak, R.Fudala, I.Gryczynski, L.Kułak, S.Shah, I. E. Serdiuk, B. Grobelna, P.Arłukowicz, A.Kubicki, P.Bojarski

Dyes and Pigments. 2018 Nov;158:60-64

From AnaSpec (Eurogentec's US subsidiary) : Lys(AMCA)-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys(TAMRA)-NH2 peptide