PCR & qPCR
DNA Polymerases
DNA Polymerases
All reagent are in separated vials to allow you to tailor reaction mix to your needs.
A kit contains 3 tubes:
- Taq ® polymerase
- MgCl2
- 10X Buffer
RT-PCR
Reverse transcriptase can be done in a One-Step or a Two-Step approach
One-Step approach
- RT and PCR in a same tube
- Less contamination risks
- Less manipulation
Two-Step approach
- RT and PCR in separate tubes
- Ideal for rare sample or multiple targets
- Core kit including dNTPs
- Primers
| Diamond Taq | |
|---|---|
| Quality Controls: | Specifications: |
| Appearance | Clear, colourless solution |
| Identity (SDS-PAGE) | MW approx. 95 kDa |
| Volume activity | = 5 U/µ |
| Purity (SDS-PAGE) | > 98% |
| Performance test: PCR on λ DNA | 0.5kb; 2.5kb; 4kb fragment positive down to 5 pg |
| Ribonucleases (up to 10 U, 1h, 37°C) | Not detectable |
| Endonucleases (up to 30 U, 16h, 65°C) | Not detectable |
| Exonucleases (up to 30 U, 16h, 65°C) | Not detectable |
| Nicking activity (up to 30 U, 16h, 65°C) | Not detectable |
| Nicking activity (up to 30 U, 16h, 65°C) | Not detectable |
| E.coli residual DNA | − 1 fg/Taq Unit |
| Bioburden | ≤ 10 CFU/ml |
| Stability | 24 months (at -20°C) from date of manufacture |
| Animal-derived additives | None |