Quenchers
QXL® - Efficient proprietary Quenchers
Quenchers are frequently used in tandem with fluorescent dyes to generate FRET pairs. The most classical FRET pairs are Mca/Dnp and EDANS/DABCYL, both emitting in the blue channel.
Their short absorption wavelength and low extinction coefficient limit their use as several proteomic samples show background autofluorescence in the blue wavelengths.
Due to the high interest in developing FRET pairs with longer emission wavelengths, AnaSpec (Eurogentec's US subsidiary) has developed the QXL® range of dark quenchers. They are individually optimized to pair with all popular fluorescent dyes such as fluoresceins and rhodamines as well as our superior HiLyte™ fluor dyes.
Our line of QXL® cover the full visible spectrum with unusually high efficiency.
Our dye/quencher labeling offer
We offer a full-range of dye-quencher FRET pairs and fluorogenic substrates.
The tables below list the dyes and quenchers available to label your custom peptides
| FRET Pairs | ||
|---|---|---|
| Dye (donor) | Quencher (acceptor) | Donor Ex/Em |
| Mca | Dnp |
•
325/393 nm |
| EDANS | DABCYL, DABCYL Plus, QXL®490 |
•
335/493 nm |
| FAM | QXL®520 |
•
492/518 nm |
| FITC | QXL®520 |
•
494/519 nm |
| HiLyte™ Fluor 488 | QXL®520 |
•
502/527 nm |
| CyLyte Fluor 3 | QXL®570 |
•
550/564 nm |
| HiLyte™ Fluor 532 | QXL®570 |
•
545/565 nm |
| HiLyte™ Fluor 555 | QXL®570 |
•
550/566 nm |
| TAMRA | QXL®570 |
•
541/568 nm |
| Rox | QXL®610 |
•
568/591 nm |
| CyLyte Fluor 5 | QXL®670 |
•
648/663 nm |
| HiLyte™ Fluor 647 | QXL®670 |
•
650/675 nm |
| CyLyte Fluor 7 | IR-QXL® |
•
750/773 nm |
QXL®490, 570, 670 are available as stand-alone catalog products, while our most popular QXL®520 and QXL®610 are exclusively available via our custom labeling services.