Dual Labeled Oligo Synthesis
Custom qPCR Probes
As a recognized oligo synthesis company since 1985, we produce various types of custom Taqman® Probes. Thanks to our vast portfolio of fluorescent dyes and NFQ quenchers, our dual labeled probes can be used in any qPCR channels from deep blue to Infrared.
Besides classical fluorophores and quenchers such as HEX, FAM, TAMRA and BHQ®, we produce custom qPCR probes labeled with our proprietary dyes (HiLyte™ Fluor, QXL®) and an IP-friendly approach for commercialization.
Custom Double dye probes
We provide Classical and locked nucleic acid Taqman® probes for real-time qPCR.
Various fluorescent dyes are available according to the selected quencher e.g. FAM/TAMRA, HEX/EDQ, ROX/DDQII and ATTO/BHQ® couples.
qPCR MGB Probes
We produce high quality Minor Groove Binding Probes perfectly suited for patient management and diagnostic of pathogens.
Our portfolio of fluorescent dyes for MGB Eclipse™ probes covers all qPCR Channels.
Molecular Beacons
We are a licensed supplier of Molecular Beacons and offer standard, wavelength-shifting and 2' O-Me RNA Molecular Beacon probes.
Our custom synthesized Molecular Beacons are available with DABCYL or BHQ® non fluorescent quenchers. Other dyes are available upon request.
LC hybridization probes
We generate LC hybridization probes on custom request
If you are interested, do not hesitate to contact us or read more about our complex oligo service.
How to select your qPCR probe?
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Select the probe type
Dual labeled Probes are widely used because they are most efficient and offer sequence specificity SYBR® Green does not. To increase sensitivity and specificity, MGB probes are recommended. -
Select your fluorescent dye
This step is important to select the most appropriate dye according to your thermocycler. To help you in dye selection, please refer to the compatibility cycler channels versus fluorescent dyes table.
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Choose the quencher adapted to your dye
Each fluorescent dye emits at a specific Wavelength. The Best quenching result is obtained with quenchers that absorb in the same range. To select the best dye/quencher couple, you can consult the dyes & quencher compatibility table.
Primer Probe Ratio
Optimal qPCR result may require primers and probe titration or adjustment of the primer/probe ratio.
Find the best Fluorophore and Quencher pair
With our Dyes and Quenchers Table find the most appropriate couple for your equipments.
Minimum guaranteed Yield
Get an estimation of the amount of Dual labeled Probe you will receive.
At your side from research to commercialization
Oligo Grades
Our oligonucleotides can be used for research, pre-diagnostic, or diagnostic applications.
We manufacture your qPCR Probes with the appropriate quality level according to your application.
Large Scale oligo synthesis
For applications requiring large amounts of primers and probes, we produce your oligonucleotides in bulk quantities up to a few dozen grams per batch.
IP-Friendly Solution
Our selection of proprietary molecules or other dyes are suitable for commercial applications.
With our Access™ Dyes & Quenchers, benefit from a Friendly license solution incl. BHQ® and MGB-Eclipse quenchers.
Related Products and Services
Besides custom primers and probes, we also produce and deliver PCR and qPCR reagents and Kits. Our offer includes off-the-shelf products but also tailor-made solutions according to your requirements.
We synthesize oligonucleotides for research, diagnostic, and therapeutic applications.
| RUO Oligonucleotides | Configure |
|---|---|
| Diagnostic Oligonucleotides | Request a Quote |
| Access™ Dyes and Quenchers | View our offer |
We produce and deliver reagents for qPCR assays. From individual component to concentrated MasterMixes, our products are optimized to ensure a high sensitivity and reproducibility.
We produce, package and release your nucleic acid-based assays. Benefit from our expertise and innovative technologies and avoid costly investments in manufacturing equipment.
We offer a highly flexible and custom service for the preparation and the delivery of your Lab Developed Tests (LDTs) in a ready-to-use format.