Why choose ATTO dyes for quantitative and digital PCR probes?

What are their primary advantages?

1. Enhanced signal stability

ATTO dyes are designed with a rigid chromophore structure (such as rhodamine, acridine, and more) that enhances photostability and reduces photobleaching. This unique feature preserves fluorescence integrity during prolonged illumination, ensuring consistent signal output in qPCR and dPCR workflows. Such stability is critical for accurate quantification, especially in assays requiring extended fluorescence monitoring.

Examples

Figure 1 : a. ATTO 495 derived from Acridine; b. ATTO 590 derived from Rhodamine; c. ATTO 647N derived from Carbopyronin

2. Bright and efficient signal intensity

For qPCR applications, signal intensity directly impacts sensitivity and detection. ATTO dyes deliver bright and efficient fluorescence, enabling precise detection of low-abundance targets. This characteristic is particularly beneficial in diagnostic assays where even the smallest signal variations matter.

3. High coupling efficiency for reliable probe labeling

One of the standout features of ATTO dyes is their high coupling efficiency, which ensures effective attachment to oligonucleotides. This reliability reduces material waste and supports cost-effective probe synthesis, especially for large-scale qPCR and dPCR projects. Efficient probe labeling enhances reproducibility, a cornerstone of reliable molecular assays.

4. Broad wavelength range

ATTO dyes are highly adaptable fluorophores that span a wide range of visible and near-infrared wavelengths. This property makes them well-suited for qPCR experiments. Indeed, with a dye available for each qPCR channel, they integrate seamlessly with various thermocyclers, ensuring compatibility across numerous platforms. This versatility makes them an ideal and easy choice for your nucleic acid based assays.

Careful consideration of dye compatibility with detection channels is essential when planning qPCR assays, as it plays a pivotal role in achieving accurate and consistent results. ATTO dyes offer the reliability and flexibility necessary for supporting a broad spectrum of qPCR applications, positioning them as a trusted option for researchers.

5. Thermostability for durable performance in qPCR cycles

qPCR assays involve repeated thermal cycling, which can degrade some dyes. ATTO dyes are thermostable, maintaining their structural integrity and fluorescence consistency across numerous cycles. This durability ensures reliable performance, even in challenging thermal conditions, making them ideal for both qPCR and dPCR applications.

Conclusion: ATTO Dyes for superior qPCR and dPCR performance

ATTO dyes excel in qPCR and dPCR applications due to their exceptional photostability, brightness, and thermostability. Their high coupling efficiency, broad wavelength range, and reliable performance make them a trusted solution for creating high-quality probes in molecular research and diagnostics. For scientists seeking precision, durability, and reproducibility, ATTO dyes are an indispensable tool in advancing assay performance.

Interested? Learn more about our ATTO-labeled probes.

Sources

1. Santos, E. M., et al. (2021). Design of large Stokes shift fluorescent proteins. J. Am. Chem. Soc., 143(38), 15599–15611. doi: 10.1021/jacs.1c05039


2. ATTO-Tec GmbH