Takyon® Amplification Reagents
SYBR® qPCR Blue Master Mix
The Blue inert dye of the Takyon® SYBR® Master Mixes is a game-changer for performance and ease of use. It will optimize your experimental workflows and enhance precision upon plate loading.
Versatile
Work across a broad spectrum of sample types, applications and compatible with any Real-Time PCR thermocycler.
Consistent
Optimized formulation for reproducible results across multiple qPCR reactions.
Convenient
Blue inert dye to simplify the experimental setup process, to reduce the risk of pipetting errors, and to save valuable time by allowing quick visual confirmation of proper plate loading.
Blue Dye: Revolutionize
your qPCR Setup
Takyon® Blue Additive is a non-fluorescent inert dye formulated to increase the contrast between qPCR Master Mixes and plasticware, thereby facilitating rapid and accurate verification of reagent distribution within the reaction wells.
Beyond its visual property, our Takyon® Blue Additive offers a multifaceted approach to enhancing both experimental performance and user experience.
With Blue Additive, you can confidently confirm proper Master Mix dispensing at a glance, minimizing the risk of human error and ensuring consistency across experiments.
On top of that, you will benefit from improved signals on Real-Time PCR platforms requiring ROX normalization.
Fast and Easy qPCR Protocol
Transitioning to Blue Master Mix is seamless, requiring no significant adjustments to
existing protocols while yielding maximal benefits in terms of visualization and ease of use.
| Steps | T°C | Time |
|---|---|---|
| Takyon® Activation | 95°C | 3 min. |
| 40 Cycles | ||
| Denaturation 2 | 95°C | 3 sec. |
| Annealing / Extension 3 | 60°C | 20-30 sec. |
1 Conditions for short amplicons (more than 120 bp). For longer amplicons or difficult templates, increase the annealing-extension time up to 40 sec.
2 Complex samples (plant DNA, Genomic DNA,...) may require a longer denaturation time.
3 The annealing temperature will vary depending on the melting temperature (Tm) of the primers.
Need even more flexibility?
Use the Core Kit version of the Blue SYBR® qPCR Kit. Not only cost-effective, it allows you to adjust each component individually and optimize your qPCR assay efficiently.
