Amplification Reagents
Reagents for quantitative
real-time PCR
Eurogentec Takyon® qPCR Kits offer fast delivery of sensitive and reproducible results for experiments. These kits include real-time PCR master mixes for both SYBR® and probe-based assays. Takyon® qPCR Kits are compatible with any qPCR thermocyclers.
With Eurogentec Takyon® qPCR Kits, you can confidently perform qualitative or quantitative real-time PCR assays and obtain accurate and reliable data.
Universal capabilities
The Takyon® kits are compatible with all cyclers and applications including high throughput and multiplex testing.
Large dynamic range
The Takyon® kits give consistent amplification results across a large range of initial target concentrations.
Superior Sensitivity
The optimized formulation of the Takyon® Kits allows for the clear detection of low copy target.
Efficient & Robust formulation
The fusion polymerase is highly efficient and less sensitive to inhibitors. This allows amplification with some difficult templates like biological fluids and viruses.
Small reaction volumes
The high sensitivity of the Takyon® kits combined with the highly concentrated 5X format gives reliable results even in small volume reaction.
Suitable for multiplex
The Takyon® kits allow for the detection of multiple targets in a single reaction tube.
SYBR® green qPCR Kits
SYBR® green is an intercalating dye used in qPCR assays. SYBR® green fluoresces only when bound to double-stranded DNA (dsDNA).
Hence the intensity of the signal from the fluorescent reporter increases proportionally to the dsDNA concentration during the amplification.
2X Takyon® SYBR® Green real-time PCR master mixes and core kits are available for any thermal cycler. They deliver sensitive, specific, and reproducible results.
Probe-based qPCR Kits
These kits use Double-dye probes such as TaqMan® Probes, MGB Probes , Molecular Beacons as a fluorescent reporter.
In Taqman® Assays, the probe specifically binds to a single DNA strand during the annealing PCR step. During the nucleic acid elongation step, the 5’ nuclease activity of the DNA polymerase cleaves the probe. The fluorescent dye is separated from the quencher and generates a fluorescent signal. The signal accumulates along the PCR cycles, proportionnaly to the initial amount of target.
The Takyon® Probe master mix and core kit have been optimized to provide high specificity, sensitivity and accuracy.
qPCR for gene expression
Check out our One-Step Takyon® Ultra Probe 4X Master Mix and combine the reverse transcription of RNA and the amplification of the cDNA, into a single tube.
Upgrade your Takyon® qPCR Kits with different options
Visual Aid
The visualization option relies on the inert blue dye that can be added into any of our clear mixes. This dye eases the visualization of dispensing errors at a glance. The contrast is even better when using white plates.
All of our Takyon® SYBR® fast qPCR master mixes and core kits as well as the most of our Probe-based kits already include the blue dye.
Blue additive is sold separately to convert any clear mixes.
Carryover Prevention
The UNG eliminates any trace of DNA molecules containing dUTP from previous reactions.
dUTP/UNG additive can be purchased separately to convert any dTTP MasterMix into a carryover prevention compatible kit.
Please note that this option is not compatible with One-Step kits.
One-step Conversion
Takyon™ One-Step Kit Converter converts any dTTP MasterMix into a One-Step qPCR Kit
Please note that this option is not compatible with One-Step kits.
