Frequently asked questions
Nucleic acids extraction & purification
Nucleic acids extraction & purification
What samples can be used with the SmartExtract DNA Extraction kit ?
The SmartExtract DNA Extraction kit achieves genomic DNA extraction from almost any crude sample type (e.g. blood, human & animal tissues, plant seeds & leaves…).
How long does it takes to recover DNA with the SmartExtract DNA Extraction kit ?
DNA extraction is performed in a single tube in 12 minutes only.
What samples can be used with the SmartPURE DNA Purification kits ?
There are 3 kits available according to the sample source.
We recommend to use the SmartPure Gel Kit for DNA purification from standard high or low melting agarose gel.
For DNA purification from PCR mixes, the SmartPure PCR Kit is indicated.
For plasmid Mini-purification from bacterial cell culture, please use the SmartPure Plasmid Kit.
How long does it takes to recover DNA from Agarose Gel with the SmartPURE Gel Kit ?
DNA purification from agarose gel takes on average 16 min.
How long does it takes to recover DNA from PCR Mixes with the SmartPURE PCR Kit ?
DNA purification from PCR Mixes takes on average 6 min.
How long does it takes to isolate and purify Plasmid from bacterial cell culture with the SmartPURE Plasmid Kit ?
Purified plasmid can be collected in 20 min on average.
What is the typical yield of plasmid recovery with the SmartPURE Plasmid Kit?
Yield may vary according to the plasmid copy number and the culture OD but the typical yield obtained is up to 40 µg plasmid DNA/1-5 mL E.coli culture.
What is the DNA size range covered by the SmartPURE Gel Kit ?
The SmartPURE Gel Kit can perform extraction and purification of DNA sequence from 60 bp to 23kbp.
What is the DNA size range covered by the SmartPURE PCR Kit ?
The SmartPURE PCR Kit can perform extraction and purification of DNA sequence from 60 bp to 10kbp.