Frequently asked questions
Labeling and Dyes
Labeling and Dyes
What is the recommended protein concentration when using an AnaTag labeling kit?
A concentration of ≥ 2mg/mL is recommended.
In which buffer can my protein be dissolved in when using AnaTag kits?
See the product data sheet for recommended buffers. It is important to avoid buffer additive reducing agents (e.g. DTT), protein stabilizers (e.g. BSA) or sodium azide. Additionally, the following buffers should be avoided: tris, glycine, ammonium salts and buffers containing free amines.
How can free dyes be removed from my labeled protein?
The free dyes are removed during the purification step via the spin column that is provided with the kit.
What purification yields can I expect?
On average, we observe 70-80% yields.
After labeling my protein, can I use a dialysis membrane to remove excess dye?
Yes, but it is important to use a membrane molecular weight cut off that is small enough to prevent protein from being lost through the membrane pores.
Where does the binding occur between the dye and my protein?
Dyes in AnaTag kits have a succinimidyl ester reactive group which can bind to any free amine available on your protein. Hence the dye can bind to any Lysine residues and protein N-terminus.