Image of a kit SensoLyte HABA Biotin Quantitation Kit Colorimetric
Assay-kits

SensoLyte® HABA Biotin Quantitation Kit Colorimetric - 1 kit

€215.00
Check your price
  • Cat.Number : AS-72096-120
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices

Quantity

Add to wishlist

The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4’-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.

Specifications

Packaging
Kits components
  • Component A: Avidin: 1 bottle Component B: HABA assay buffer: 25 mL Component C: d-biotin: 100 µM, 200 µL
Properties
Absorbance (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2-16uM
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

Downloads

You may also be interested in the following product(s)

Image of a kit AnaTag Biotin Protein Labeling

AnaTag™ Biotin Protein Labeling Kit - 1 kit

Cat.Number : AS-72057
€324.00 Excl. Tax
Bottle of Protein A-Biotin Conjugate

Protein A-Biotin Conjugate - 5 mg

Cat.Number : AS-72234
€214.00 Excl. Tax