Labeling-detection

Protein A-Biotin Conjugate - 5 mg

214.00
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  • Cat.Number : AS-72234
  • Availability :
    In stock
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Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M).
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in Staphylococcus aureus. Biotinylated Protein A can be used for assay development, IHC, IF, immunoprecipitation assays, and for western blotting.

Specifications

Chemistry
Molecular Mass/ Weight
  • ~30000
Quantity & Purity
Concentration
  • 1 mg/ml
Purity
  • >95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 10mM phosphate, 150mM sodium chloride, pH 7.2, with 0.1% sodium azide as preservative
Storage Conditions
  • Stable for 2-3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles.
Activity
Application
Biomarker Target
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • E. coli
Codes
Code Nacres
  • NA.32

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