Image of a kit SensoLyte Anti-Mouse/Rat Beta-Amyloid (1-42) Quantitative ELISA Kit Colorimetric
Assay-kits

SensoLyte® Anti-Mouse/Rat ß-Amyloid (1-42) Quantitative ELISA Kit Colorimetric - 1 kit

€594.00
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  • Cat.Number : AS-55554
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This SensoLyte® high-sensitivity (2pg/ml) beta-Amyloid (1-42) Quantitative ELISA Kit (Mouse/Rat) provides a convenient and quantitative assay for determining mouse/rat beta-Amyloid (1-42) (Aβ42) amount in cell and tissue lysate as well as in body fluids. Compared to other mouse/rat anti-Aβ42 ELISA kits on the market, it takes less time to run this assay. HRP conjugated detection antibody in this kit is added simultaneously with the samples and standards during the assay. This eliminates extra incubation and washing steps and makes this kit a one-step procedure for Aβ42 quantification.

Alzheimer's Disease (AD) is the most common neurodegenerative disorder in elderly people. It has been demonstrated that AD has biological causes and is characterized by the presence of senile plaques and neurofibrillary tangles mainly in cerebral cortex and hippocampus brain regions. Beta-Amyloid (1-40) (Aβ40) and beta-Amyloid (1-42) (Aβ42) are the main components of the above plaques; however, other forms of beta-Amyloid peptides are also present. Both peptides are cleaved from the Amyloid Precursor Protein (APP) by β-secretase and γ-secretase enzymes. Many studies suggest that Aβ42 or/and Aβ43 are required to initiate formation of amyloid plaques and neurofibrills that leads to the neurodegeneration, while Aβ40 is less neurotoxic.

Specifications

Packaging
Kits components
  • Component A: Mouse monoclonal anti-beta-Amyloid (1-42) coated plate: 12 x 8-well strips. Component B: Mouse/rat beta-Amyloid (1-42) Peptide Standard (lyophilized): 1 µg. Component C: 1 X Sample Dilution Buffer: 60 ml. Component D: 10 X Wash Buffer: 30 ml. Component E: TMB Color Substrate Solution: 10 ml. Component F: Stop Solution: 10 ml. Component G: Peptide Standard Reconstitution Buffer: 1 ml. Component H: Detection Antibody (Rabbit anti-Mouse/Rat beta-Amyloid-HRP): 50 µl. Component I: 1 X Detection Antibody Dilution Buffer: 10 ml. Component J: Adhesive Plate Covers: 2 pieces.
Chemistry
UniProt number
  • P12023, P08592
Storage & stability
Storage Conditions
  • Store all components at 4°C for up to 6 months. Component B Reconstituted Peptide Standard must be stored at -80 °C in small aliquots. Protect components E and G from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Mouse/Rat
Codes
Code Nacres
  • NA.32

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Citations

Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's Disease.

J Neurosci . 2014 Nov 05 ; 34 14919 | DOI : 10.1523/JNEUROSCI.1165-14.2014.

  • J. Zhang
  • et al