SensoLyte® 520 IDE Activity Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-72231
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Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme. Growing evidences have indicated that IDE may be involved in the pathogenesis of Alzheimer’s disease and diabetes mellitus type 2.
The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.
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Citations
Acute exercise restores insulin clearance in diet-induced obese mice
J Endocrinol . 2016 Mar 21 ; 229 221-32 | DOI : 10.1530/JOE-15-0483
- M.A. Kurauti
- et al
Hyperinsulinemia caused by dexamethasone treatment is associated with reduced insulin clearance and lower hepatic activity of insulin-degrading enzyme
J Steroid Biochem Mol Biol . 2015 Sep 16 ; 155(Pt A) 1-8 | DOI : 10.1016/j.jsbmb.2015.09.020
- A.O. Protzek
- et al
Piceatannol, a natural analogue of resveratrol, effectively reduces beta-amyloid levels via activation of alpha-secretase and matrix metalloproteinase-9
J Func Foods . 2016 May 01 ; 23 124 | DOI : https://doi.org/10.1016/j.jff.2016.02.024
- C. Boyoung
- et al
Effect of trichostatin A on gelsolin levels, proteolysis of amyloid precursor protein, and amyloid beta-protein load in the brain of transgenic mouse model of Alzheimer's disease
Curr Alzheimer Res . 2014 Dec 01 ; 11 1002-11 | DOI : 10.2174/1567205011666141107125531
- W. Yang
- et al
Effects of Fasting and Feeding on Transcriptional and Posttranscriptional Regulation of Insulin-Degrading Enzyme in Mice.
Cells . 2021 Sep 16 ; 10(9) 2446 | DOI : 10.3390/cells10092446
- CM González-Casimiro
- et al