Tube of HL647-T10
PCR & qPCR

HL647-T10 Calibration Oligo - 5 nmol

€16.00
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  • Cat.Number : UN-CT030-005
  • Availability :
    In production

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Dye-labeled calibration oligos are 5’ fluorescent 10-mers dT oligonucleotides used as a reference to calibrate real-time qPCR thermocyclers.

Calibration is a preliminary step meant to adjust spectral profile for each single dye on the qPCR platforms. This allows to compensate for crosstalks between dyes (channels) in the context of multiplexed assays, which is critical for appropriate result analysis.

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Specifications

Packaging
Kits components
  • 1 x 2 mL tube containing 5 nmol of calibrating oligo
Chemistry
Sequence one letter code
  • 5' HiLyte™ Fluor 647 - TTT-TTT-TTT-T 3'
Linkage
  • Phosphodiester
Molecular Mass/ Weight
  • 4372.7
Properties
Absorbance (nm)
  • 650
Emission (nm)
  • 675
Color
Modification
Modification Position
  • 5'
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purification Method
  • RP-HPLC
Purity
  • >85%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • To make up to 100 µM concentration, add 50 µl of recommended buffer. To make up to 20 µM concentration, add 250 µl of recommended buffer. To make up to 5 µM concentration, add 1000 µl of recommended buffer.
Storage Buffer
  • TE buffer (pH 8) or dH2O
Storage Conditions
  • Short-term storage: Room temperature Long-term storage: -20°C After reconstitution in appropriate buffer: -20°C for 12 months
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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