PCR & qPCR

Takyon™ ROX Probe 2X MasterMix dTTP blue

382.00
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  • Cat.Number : UF-RPMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Size

Alternative choices

Quantity

Takyon™ qPCR Kits for Probe assays containing ROX passive reference are available in different MasterMix formats from 1.5 ml to 10 x 7.5 ml and Core Kits. Takyon™ qPCR Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & regular cycling conditions.

To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter (UF-RTAD-D0101).

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (blue cap - amber tube) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (blue cap - amber vial) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ Rox Probe MasterMix dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon™ Rox Probe MasterMix dTTP blue can be stored at 4 °C for 6 months.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Normoxic induction of HIF-1α by adenosine-A2BR signaling in epicardial stromal cells formed after myocardial infarction

FASEB . 2021 Apr 29 ; (2021) 35 1-12 | DOI : https://doi.org/10.1096/fj.202002545R

  • Julia Hesse
  • et al