Biotin Assays

Convenient Biotin quantitation kits

The SensoLyte® Biotin Assay Kits provide a convenient assay for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantifying biotin concentration in a solution.

The SensoLyte® Biotin Quantitation Kit is based on a FRET assay:

• First, avidin (Reagent A tagged with a quencher dye) is briefly incubated with biotin samples/standards.
• Second, fluorescent biotin (Reagent B) is added to the assay mixture. The fluorescent signal is directly proportional to the amount of biotin in the sample and can be detected at Ex/Em=490/520 nm.

The HABA Biotin Quantitation assay employs HABA (4’-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.