How to optimize a qPCR assay?

If the primers and Double-Dye Oligo have been designed using Primer Express™ and are used in combination with the qPCR MasterMix or qPCR MasterMix for SYBR

Cytoprotective effects of α-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, oleic acid and α-tocopherol on 7-ketocholesterol –Induced oxiapoptophagy: Major roles of PI3-K / PDK-1 / Akt signaling pathway and glutathione peroxidase activity in cell rescue

Aline Yammine, Imen Ghzaiel, Vivien Pires, Amira Zarrouk, Omar Kharoubi, Hélène Greige-Gerges, Lizette Auezova, Gérard Lizard, Anne Vejux Takyon™ ROX SYBR

RT-RTCK-03 Reverse Transcriptase Core Kit 300 (EN)

For Europe: ® E-mail: info@eurogentec.com 4.2- In case of a SYBR green I assay Tel : +32 4 372 76 65 Component Volume (μl) Final concentration For USA:...
green I 1.5 - - HotGoldStar 0.25 - 0.025 U / μl RNase free water 16.25 5 - Total Mix 40 μl ® * If using a qPCR Core kit for SYBR green I ® ** If using...
a qPCR MasterMix Plus for SYBR green I Add 40 μl of PCR reaction Mix to 10 μl of the 1rst strand reaction mix, or a dilution of it. 4.3- To correct for

RT-RTCK-05 Reverse Transcriptase Core Kit 500 (EN)

For Europe: ® E-mail: info@eurogentec.com 4.2- In case of a SYBR green I assay Tel : +32 4 372 76 65 Component Volume (μl) Final concentration For USA:...
green I 1.5 - - HotGoldStar 0.25 - 0.025 U / μl RNase free water 16.25 5 - Total Mix 40 μl ® * If using a qPCR Core kit for SYBR green I ® ** If using...
a qPCR MasterMix Plus for SYBR green I Add 40 μl of PCR reaction Mix to 10 μl of the 1rst strand reaction mix, or a dilution of it. 4.3- To correct for

UF-LPMU-C0701 2XRB Takyon LO ROX Probe MMx UNG (EN)

Identification of the substance/mixture and of the company/undertaking 1.1 Product details · Component name 2x Reaction Buffer · Product name Takyon Low ROX SYBR...
® MMx dTTP blue 1,5ml Takyon Low ROX SYBR® MMx dTTP blue 7,5ml · Catalog number UF-LSMT-B0101 UF-LSMT-B0701 1.2 Relevant identified uses of the substance...
Takyon™ is a trademark of Kaneka Eurogentec S.A. ® GoldStar is a registered trademark of Kaneka Eurogentec SA. ® SYBR is a registered trademark of Molecular

Gene Synthesis, Analysis and Delivery Brochure (EN)

EtBr, SYBR Safe, ® > No risk of DNA decay SYBR Gold, SYBR Green I & II, > User Friendly ® ® The blue LEDs of the Smart SYPRO Ruby, SYPRO Orange, Easy to...
Finalise your order trademarks labels ® MapMarker is a registered trademark of BioVentures, Inc. ® Mupid is a registered trademark of Advance Co., Ltd ® SYBR

PCR and qPCR Brochure (EN)

Assays ® 2 Takyon™ ROX SYBR MasterMix 2X Blue dTTP 7.5 mL UF-RSMT-B0701 UF-RSMT-B0705 UF-RSMT-B0710 750 MasterMix 2x UF-RSMT-B0100 ® 2 Takyon™ ROX SYBR...
® 2 Takyon™ No ROX SYBR Core Kit blue dTTP UF-NSCT-B0201 UF-NSCT-B0205 UF-NSCT-B0210 1250 Core Kit ® 2 Takyon™ SYBR 2X MasterMix blue dTTP with fluorescein...
SYBR® kit).

Controls – PCR and qPCR | Eurogentec

Benefits : Validated for human samples Sensitive and specific detection Full expression range coverage Compatible with SYBR® green or probe Assay Each

50mM MGCL2 (EN)

Product identifier For the following kits : MESA GREEN/BLUE/FAST MasterMixes ; Takyon™ qPCR and RT-qPCR MasterMixes and Core kits for SYBR® and Probe assays...
; qPCR and RT-qPCR MasterMixes Plus and Core Kits for SYBR® and Probe assays....
SYBR® Green is a registered trademark of Life Technologies Corp.

5mM dNTP Mix (EN)

substance/mixture and of the company/undertaking 1.1 Product details · Component name 5mM dNTP Mix · Product name qPCR Core kit no dUTP ® qPCR Core kit for SYBR...
Takyon™ is a trademark of Kaneka Eurogentec S.A. ® GoldStar is a registered trademark of Kaneka Eurogentec SA. ® SYBR is a registered trademark of Molecular

qPCR Glossary

SYBR® Green: SYBR® Green is an inexpensive, universal dye which binds to dsDNA.

RT-PARE Rox Passive Reference for qPCR use (EN)

SYBR Green I nucleic acid gel stain ® iCycler is a registered trademark of Bio-Rad Laboratories, Inc.

Trademarks Labels | Eurogentec

SYBR® Green is a registered trademark of Life Technologies Corp. Taqman® is a registered trademark of Roche Diagnostics GmbH.

RT-RTCK Reverse Transcriptase Core kit (EN)

. ® SYBR is a registered trademark of Molecular Probes Inc.

RT-RTCK-05 RNase Free Water (EN)

. ® SYBR is a registered trademark of Molecular Probes Inc.

RT-CKFT-23S E coli Xsomal DNA (EN)

. ® SYBR is a registered trademark of Molecular Probes Inc.

5XRB TAKYON Probe MMX (EN)

. ® SYBR is a registered trademark of Molecular Probes, Inc.

UF-NPCT-C0201 2XRB Takyon No Rox Probe Core kit dTTP (EN)

GoldStar ® SYBR is a registered trademark of Molecular Probes, Inc.

UF-NPMU-C0701 2XRB Takyon No ROX Probe MMx UNG (EN)

. ® SYBR is a registered trademark of Molecular Probes, Inc.

UF-RPMU-C0701 2XRB Takyon ROX Probe MMx UNG (EN)

. ® SYBR is a registered trademark of Molecular Probes, Inc.