qPCR Guide (EN)

These specific techniques can be used to multiplex the assay, i.e. detecting several different targets in the same assay. ® SYBR Green I ® SYBR Green I...
Green I assays ® As SYBR Green I binds to any dsDNA it is important to avoid primer-dimers and non-specific ® products in SYBR Green I assays....
Detection using SYBR Green I on a LC480 thermocycler.

RT-qPCR Sample Test Guide (EN)

Mix) or 5.5 mM (Probe Kits) MgCl . 2 Differences in MgCl concentrations between two kits should be considered, 2 ® 2+ particularly when performing a SYBR...
Ideally, the comparative assay should include the same or a comparable inert dye. ® ® The Takyon Blue dye even helps to increase signal intensity in SYBR...
Detection using SYBR 22.5 Green I on a QuantStudio™ 5 thermocycler. 20.0 17.5 15.0 12.5 10.0 0.010.02 0.1 0.2 122 3415 10 2030 100 00 1000 10000 00000

Technical information for Takyon® qPCR assays (EN)

eurogentec.com Complementary information for qPCR assays : Takyon® SYBR® assays Primer design guidelines – GC content should be between 30 % and 80 % (...
Table 1 : Primer titration Reverse Forward ® matrix for SYBR assays 50 nM 100 nM 300 nM 50 nM 50 / 50 100 / 50 300 / 50 100 nM 50 / 100 100 / 100 300 /...
Bois Saint-Jean, 5 T +32 4 372 74 00 info@eurogentec.com 4102 Seraing - Belgium F +32 4 372 75 00 www.eurogentec.com RES-TEC-EN-Technical information SYBR-NOV24

HotGoldStar (EN)

the substance/mixture and of the company/undertaking 1.1 Product details · Component name HotGoldStar · Product name qPCR Core kit qPCR Core kit for SYBR...
® green I qPCR Core kit No ROX qPCR Core kit for SYBR® green I No ROX qPCR Core kit No dUTP qPCR Core Kit for SYBR® green I No dUTP qPCR Core kit Only...
dUTP qPCR Core kit for SYBR® green I Only dUTP · Catalog number RT-QP73-05 RT-QP73-01 RT-SN73-05 RT-SN10-05 RT-SN73-01 RT-QP73-05NR RT-SN73-05NR RT-SN10

RT-RTCK-03 Reverse Transcriptase Core Kit 300 (EN)

For Europe: ® E-mail: info@eurogentec.com 4.2- In case of a SYBR green I assay Tel : +32 4 372 76 65 Component Volume (μl) Final concentration For USA:...
green I 1.5 - - HotGoldStar 0.25 - 0.025 U / μl RNase free water 16.25 5 - Total Mix 40 μl ® * If using a qPCR Core kit for SYBR green I ® ** If using...
a qPCR MasterMix Plus for SYBR green I Add 40 μl of PCR reaction Mix to 10 μl of the 1rst strand reaction mix, or a dilution of it. 4.3- To correct for

RT-RTCK-05 Reverse Transcriptase Core Kit 500 (EN)

For Europe: ® E-mail: info@eurogentec.com 4.2- In case of a SYBR green I assay Tel : +32 4 372 76 65 Component Volume (μl) Final concentration For USA:...
green I 1.5 - - HotGoldStar 0.25 - 0.025 U / μl RNase free water 16.25 5 - Total Mix 40 μl ® * If using a qPCR Core kit for SYBR green I ® ** If using...
a qPCR MasterMix Plus for SYBR green I Add 40 μl of PCR reaction Mix to 10 μl of the 1rst strand reaction mix, or a dilution of it. 4.3- To correct for

PCR and qPCR Brochure (EN)

Assays ® ® 2 Takyon ROX SYBR MasterMix 2X Blue dTTP 7.5 mL UF-RSMT-B0701 UF-RSMT-B0705 UF-RSMT-B0710 750 MasterMix 2x UF-RSMT-B0100 ® ® 2 Takyon ROX SYBR...
2x UF-NSMT-B0100 ® ® 2 Takyon No ROX SYBR Core Kit blue dTTP UF-NSCT-B0201 UF-NSCT-B0205 UF-NSCT-B0210 1250 Core Kit ® ® 2 Takyon SYBR 2X MasterMix blue...
SYBR kit). Transcriptase Core Kit prior the qPCR assay to make a two steps reactions with any qPCR kit.

Gene Synthesis, Analysis and Delivery Brochure (EN)

EtBr, SYBR Safe, ® > No risk of DNA decay SYBR Gold, SYBR Green I & II, > User Friendly ® ® The blue LEDs of the Smart SYPRO Ruby, SYPRO Orange, Easy to...
Finalise your order trademarks labels ® MapMarker is a registered trademark of BioVentures, Inc. ® Mupid is a registered trademark of Advance Co., Ltd ® SYBR

50mM MGCL2 (EN)

Product identifier For the following kits : MESA GREEN/BLUE/FAST MasterMixes ; Takyon™ qPCR and RT-qPCR MasterMixes and Core kits for SYBR® and Probe assays...
; qPCR and RT-qPCR MasterMixes Plus and Core Kits for SYBR® and Probe assays....
SYBR® Green is a registered trademark of Life Technologies Corp.

RT-PARE Rox Passive Reference for qPCR use (EN)

SYBR Green I nucleic acid gel stain ® iCycler is a registered trademark of Bio-Rad Laboratories, Inc.

RT-RTCK-05 RNase Free Water (EN)

. ® SYBR is a registered trademark of Molecular Probes Inc.

RT-RTCK Reverse Transcriptase Core kit (EN)

. ® SYBR is a registered trademark of Molecular Probes Inc.

Oligonucleotides Brochure (EN)

. ® SYBR Green is a registered ® MGB Eclipse is a registered ® Cascade Blue is a registered trademark of Life Technologies trademark of ElitechGroup, Inc