Image of a kit Takyon One-Step Rox Probe 5X MMx 1250rxn
PCR & qPCR

Takyon™ One-Step Rox Probe 5X MasterMix dTTP

$1,464.00
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  • Cat.Number : UF-RP5X-RT0501
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    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ One-Step 5X qRT-PCR Kits for Probe assays containing ROX passive reference are available in different MasterMix formats from 0.6 ml to 10 x 5 ml. Takyon™ 5X qRT-PCR Kits deliver FAST, SENSITIVE and ACCURATE results in fast & regular cycling conditions

These kits are perfectly suited for gene expression studies and demonstrate exceptional performance on viral templates thanks to the newly developped EuroscriptII RT
Moreover the new additive allows a significant improvement of the performance on some difficult virus types as well as on FFPE samples.

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (0.6 mL) of 5x MasterMix (blue cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 30 µL of Euroscript II RT (50 u/µL) / RNAse inhibitor(20u/µL) (white cap) 30 µL of Additive (blue cap) that improve results on some viral and FFPE samples 1250 reactions kit contains each: Five tubes (1 mL) of 5x MasterMix (blue cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 5 X
Storage & stability
Form
  • In solution
Storage Conditions
  • The 5X MasterMix components of the Takyon™ One-Step Rox Probe 5X MasterMix dTTP should be stored between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, this component is stable for 24 months (1 year for the RT). For short term storage the Takyon™ One-Step Rox Probe 5X MasterMix dTTP can be stored at 4 °C for 6 months. - Euroscript II RT & additive should be stored at a temperature between -65°C and -75°C in a constant temperature freezer. When stored under these conditions, the reagents are stable for 1 year. For short term storage the Euroscript II RT and additive can be stored between -15°C and -25°C for 1 month.
Activity
qPCR compatible thermocyclers
Application
Template type
  • RNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Clinical and in Vitro Evidence against Placenta Infection at Term by Severe Acute Respiratory Syndrome Coronavirus 2

ScienceDirect . 2021 Jun 08 ; 9 1610-1623 | DOI : https://doi.org/10.1016/j.ajpath.2021.05.009

  • Arthur Colson
  • et al