Image of a kit Takyon One-Step No Rox Probe 5X 1250 rxn
PCR & qPCR

Takyon™ One-Step No Rox Probe 5X MasterMix dTTP

€1,240.00
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  • Cat.Number : UF-NP5X-RT0501
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ One-Step 5X qRT-PCR Kits for Probe assays containing no ROX passive reference are available in different MasterMix formats from 0.6 ml to 10 x 5 ml. Takyon™ 5X qRT-PCR Kits deliver FAST, SENSITIVE and ACCURATE results in fast & regular cycling conditions.

These kits are perfectly suited for gene expression studies and demonstrate exceptional performance on viral templates thanks to the newly developped EuroscriptII RT
Moreover the new additive allows a significant improvement of the performance on some difficult virus types as well as on FFPE samples.

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (0.6 mL) of 5x MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 30 µL of Euroscript II RT (50 u/µL) / RNAse inhibitor(20u/µL) (white cap) 30 µL of Additive (blue cap) that improve results on some viral and FFPE samples 1250 reactions kit contains each: Five tubes (1 mL) of 5x MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 250 µL of Euroscript II RT (50 u/µL) / RNAse inhibitor(20u/µL) (white cap) 250 µL of Additive (blue cap) that improve results on some viral and FFPE samples
Properties
Format
Color
Quantity & Purity
Concentration
  • 5 X
Storage & stability
Form
  • In solution
Storage Conditions
  • 5X MasterMix components of the Takyon™ One-Step No Rox 5X Probe MasterMix dTTP should be stored between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, this component is stable for 24 months. For short term storage the Takyon™ One-Step No Rox Probe 5X MasterMix dTTP can be stored at 4 °C for 6 months. - Euroscript II RT & additive should be stored at a temperature between -65°C and -75°C in a constant temperature freezer. When stored under these conditions, the reagents are stable for 1 year. For short term storage the Euroscript II RT and additive can be stored between -15°C and -25°C for 1 month
Activity
qPCR compatible thermocyclers
Application
Template type
  • RNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

Methods Protoc . 2020 Aug 18 ; 3(3), 59 | DOI : doi.org/10.3390/mps3030059

  • Damien Coupeau
  • Nicolas Burton
  • Noemie Lejeune
  • Suzanne Loret
  • Astrid Petit
  • Srdan Pejakovic
  • Florian Poulain
  • Laura Bonil
  • Gabrielle Trozzi
  • Laetitia Wiggers
  • Kevin Willemart
  • Emmanuel Andre
  • Lies Laenen
  • Lize Cuypers
  • Marc Van Ranst
  • Pierre Bogaerts
  • Benoit Muylkens
  • Nicolas Albert Gillet

SARS-CoV-2 Surveillance in Belgian Wastewaters

Viruses . 2022 Sep 02 ; 14(9) (2022) 1950 | DOI : https://doi.org/10.3390/v14091950

  • Raphael Janssens
  • et al