Image of a kit Takyon One-Step Low RoxProbe 5X 1250 rxn
PCR & qPCR

Takyon™ One-Step Low Rox Probe 5X MasterMix dTTP

€1,240.00
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  • Cat.Number : UF-LP5X-RT0501
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    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ One-Step 5X qRT-PCR Kits for Probe assays containing Low ROX passive reference are available in different MasterMix formats from 0.6 ml to 10 x 5 ml. Takyon™ 5X qRT-PCR Kits deliver FAST, SENSITIVE and ACCURATE results in fast & regular cycling conditions.

These kits are perfectly suited for gene expression studies and demonstrate exceptional performance on viral templates thanks to the newly developped EuroscriptII RT
Moreover the new additive allows a significant improvement of the performance on some difficult virus types as well as on FFPE samples.

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: 0.6 mL of 5X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Rox Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use). 250µL of Reverse tanscriptase/RNAse inhibitor (white cap) containing 50 u/µL RT and 20 u/µL RNAse inh. 250 µL of Additive (blue cap) which improves results on some viral and FFPE samples. 1250 reactions kit contains each: 5 x 1 mL of 5X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Rox Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use). 250µL of Reverse tanscriptase/RNAse inhibitor (white cap) containing 50 u/µL RT and 20 u/µL RNAse inh. 250 µL of Additive (blue cap) which improves results on some viral and FFPE samples.
Properties
Format
Color
Quantity & Purity
Concentration
  • 5 X
Storage & stability
Form
  • In solution
Storage Conditions
  • 5X MasterMix components of the Takyon™ One-Step 5X Probe MasterMix should be stored between -15 °C and -25 °C in a constant temperature freezer. When stored under these conditions, this component is stable for 24 months. For short term storage the Takyon™ One-Step Low Rox Probe 5X MasterMix dTTP can be stored at 4 °C for 6 months. Euroscript II RT & additive should be stored at a temperature between -65°C and -75°C in a constant temperature freezer. When stored under these conditions, the reagents are stable for 1 year. For short term storage the Euroscript II RT and additive can be stored between -15°C and -25°C for 1 month.
Activity
qPCR compatible thermocyclers
Application
Template type
  • RNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Removal of senescent cells reduces the viral load and attenuates pulmonary and systemic inflammation in SARS-CoV-2-infected, aged hamsters

Nat Aging . 2023 Jul 06 ; | DOI : https://doi.org/10.1038/s43587-023-00442-w

  • Lou Delval
  • et al