PCR & qPCR

Takyon™ No ROX Probe 2X MasterMix dTTP

382.00
Check your price
  • Cat.Number : UF-NPMT-C0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Size

Alternative choices

Quantity

Takyon™ qPCR Kits for Probe assays containing no ROX passive reference are available in different MasterMix formats from 1.5 ml to 10 x 7.5 ml. Takyon Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions

For carry-over prevention, combine any dTTP Mastermix with the new dUTP/UNG additive (RT-UTPUNG-020)

To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter (UF-RTAD-D0101).

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (black cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ No Rox Probe MasterMix dTTP should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon™ No Rox Probe MasterMix dTTP can be stored at 4 °C for 6 months.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Comparative Analysis of Salmon Cell Lines and Zebrafish Primary Cell Cultures Infection with the Fish Pathogen Piscirickettsia salmonis

Microorganisms . 2021 Dec 06 ; (2021) 9, 2516 1-21 | DOI : https://doi.org/10.3390/microorganisms9122516

  • Javiera Ortiz-Severín
  • et al

Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection of Leishmania spp. in Human Serum

Pathogens . 2021 Jun 30 ; 2021, 10, 826 1-11 | DOI : https://doi.org/10.3390/pathogens10070826

  • Konstantin Tanida
  • et al

Reactive microglia are the major source of tumor necrosis factor alpha and contribute to astrocyte dysfunction and acute seizures in experimental temporal lobe epilepsy

GLIA . 2022 Sep 08 ; (2022) 1–19 | DOI : https://doi.org/10.1002/glia.24265

  • Lukas Henning
  • et al

A SNP marker to discriminate the european brook lamprey (Lampetra planeri), river lamprey (L. fuviatilis) and their hybrids

Molecular Biology Reports . 2022 Sep 04 ; 49 (2022) 10115–10119 | DOI : https://doi.org/10.1007/s11033-022-07800-8

  • Ahmed Souissi
  • et al

Identification of Vietnamese Flea Species and Their Associated Microorganisms Using Morphological, Molecular, and Protein Profiling

Microorganisms . 2023 Mar 09 ; 11 (2023) 716 | DOI : https://doi.org/10.3390/microorganisms11030716

  • Ly Na Huynh
  • et al