Image of a kit Takyon Low Rox Probe Mmx dTTP, 1250 RXN
PCR & qPCR

Takyon™ Low ROX Probe 2X MasterMix dTTP

$451.00
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  • Cat.Number : UF-LPMT-C0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ qPCR MasterMixes for Probe assays containing Low ROX passive reference are available in different formats from 1.5 ml to 10 x 7.5 ml. Takyon™ Kits deliver FAST, SENSITIVE and ACCURATE results on the following FAST & regular Cycling conditions

On the Mx3000P®, Mx3005® and Mx4000®, both MasterMixes with and without ROX passive reference can be used depending on your needs. If you do not perform ROX normalisation on your results, we advise you to use the Takyon Kits for Probe Assay - No ROX which have been validated on these thermocyclers.
For carry-over prevention, combine any dTTP Mastermix with the new dUTP/UNG additive (RT-UTPUNG-020).
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ qPCR MasterMixes should be stored between -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable during 24 months. For short term storage the Takyon™ qPCR MasterMixes can be stored at 4 °C for 6 months.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Interaction of two MADS-box genes leads to growth phenotype divergence of all-flesh type of tomatoes

Nature communications . 2021 Nov 25 ; (2021) 12:6892 | DOI : https://doi.org/10.1038/s41467-021-27117-7

  • Baowen Huang
  • et al