Image of a kit SensoLyte Rh110 Factor Xa Assay Kit Fluorimetric
Assay-kits

SensoLyte® Rh110 Factor Xa Assay Kit Fluorimetric - 1 kit

€582.00
Check your price
  • Cat.Number : AS-72207
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices

Quantity

Add to wishlist

Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.
The SensoLyte® Rh110 Factor Xa Assay Kit provides a convenient assay for screening of FXa inhibitors or continuous assay of enzyme activity using a fluorogenic substrate. Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components.

Specifications

Packaging
Kits components
  • Component A: Rh110 Factor Xa substrate, Ex/Em=490 nm/520 nm upon cleavage: 0.4 mM, 50 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 0.4 mM, 10 µL Component C: Purified Bovine Factor Xa: 5 ng/µL, 20 µL Component D: 2X Assay Buffer: 25 mL Component E: Factor Xa Inhibitor: 1 mM, 20 µL
Chemistry
UniProt number
  • P00742
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.31 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

Downloads

You may also be interested in the following product(s)

Tube of PAR-4 Agonist amide peptide

Protease-Activated Receptor-4, PAR-4 Agonist, amide

Cat.Number : AS-60218-1
€88.00 Excl. Tax
Tube of TRAP-6 peptide

Thrombin Receptor Activator for Peptide 6 (TRAP-6)

Cat.Number : AS-24190
€40.00 Excl. Tax
Vial of Thrombin Substrate AFC peptide

Thrombin Substrate, fluorescent (H-D-Phe-Pro-Arg-AFC) - 10 mg

Cat.Number : AS-24131
€198.00 Excl. Tax

Citations

Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches

Molecules . 2017 Sep 22 ; 22 E1588 | DOI : 10.3390/molecules22101588

  • F.C. Lagos
  • et al