Image of a kit SensoLyte pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit Colorimetric
Assay-kits

SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit Colorimetric - 1 kit

€244.00
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  • Cat.Number : AS-72144
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The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time. SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.

Specifications

Packaging
Kits components
  • Component A: pNPP, colorimetric placental alkaline phosphatase substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Stop solution: 25 mL Component D: Triton-X-100: 500 µL Component E: Human Placental Alkaline Phosphatase Standard: 10 µg/mL, 100 µL
Properties
Absorbance (nm)
  • 405
Storage & stability
Storage Conditions
  • Store components B and E at -20°C. All other components at 4°C. Protect component A from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

The isolation of CHO cells with a site conferring a high and reproducible transgene amplification rate

J biotechnol . 2013 Feb 01 ; 164(2) 346 | DOI : 10.1016/j.jbiotec.2013.01.016

  • J.J. Cacciatore
  • et al