SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit
- Cat.Number : AS-72146
- Manufacturer Ref. :
-
Availability :
In stock
- Shipping conditions : Ice delivery fees must be applied
Alternative choices
The change of alkaline phosphatase activity is involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases and inflammatory bowel diseases. Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate.
This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.
Specifications
| Packaging | |
| Kits components |
|
|---|---|
| Properties | |
| Absorbance (nm) |
|
| Storage & stability | |
| Storage Conditions |
|
| Activity | |
| Application | |
| Biomarker Target | |
| Detection Method | |
| Detection Limit |
|
| Research Area | |
| Sub-category Research Area | |
| Usage |
|
| Codes | |
| Code Nacres |
|
Downloads
You may also be interested in the following product(s)
SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric - 1 kit
SensoLyte® Luminescent Alkaline Phosphatase Assay Kit Luminometric - 1 kit
Citations
Amelioration of the fitness costs of antibiotic resistance due to reduced outer membrane permeability by upregulation of alternative porins
MolBioEvol . 2015 Sep 09 ; 15 | DOI : 10.1093/molbev/msv195
- M. Knopp
- et al
Cabozantinib Inhibits Prostate Cancer Growth and Prevents Tumor-Induced Bone Lesions
Clin Cancer Res . 2013 Oct 04 ; 20 617 | DOI : 10.1158/1078-0432.CCR-13-0839
- J. Dai
- et al
The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo
Archives Oral Biol . 2014 Mar 02 ; 59 539 | DOI : 10.1016/j.archoralbio.2014.02.011
- Y.J. Mah
- et al
Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts
Acta Biomat . 2013 Oct 03 ; 10 439 | DOI : https://doi.org/10.1016/j.actbio.2013.09.037
- L. Pauksch
- et al
Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells
Biochim Biophys Acta -Mol Cell Biol Lipids . 2014 Jan 13 ; 1841 514 | DOI : 10.1016/j.bbalip.2014.01.002
- A. Rebollo
- et al
The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration
Biomat . 2013 Dec 30 ; 35 2446 | DOI : 10.1016/j.biomaterials.2013.12.006
- S. Yamano
- et al
Facile surface functionalization with glycosaminoglycans by direct coating with mussel adhesive protein
Tissue Eng Part C Meth . 2011 Nov 08 ; 18 71 | DOI : 10.1089/ten.TEC.2011.0384
- B. Choi
- et al
The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys
Intl J Mol Sci . 2012 Jan 23 ; 13 5098 | DOI : 10.3390/ijms13045098
- M. Kamiyama
- et al
Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold
Nanoscale Res Lett . 2012 Jan 17 ; 7 78 | DOI : 10.1186/1556-276X-7-78Open Access
- S-M. Kim
- et al
Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates
Invest Ophthalmol Vis Sci . 2012 Jan 31 ; 53 513-520 | DOI : 10.1167/iovs.11-7550
- E. Martinez-Conesa
- et al
Heat treatment of Na2O-CaO-P2O5-SiO2 bioactive glasses: Densification processes and postsintering bioactivity
J Biomed Mat Res . 2011 Nov 02 ; 100A 305 | DOI : 10.1002/jbm.a.33276
- A. Sola
- et al
Biomimetic interpenetrating polymer network hydrogels based on methacrylated alginate and collagen for 3D pre-osteoblast spreading and osteogenic differentiation
Soft Matter . 2012 Jan 12 ; 8 2398 | DOI : 10.1039/C2SM06869A
- J. Sun
- et al
Highly efficient antiviral and antibacterial activities of solid-state cuprous compounds
J Haz Mat . 2012 Aug 06 ; 265 235-236 | DOI : 10.1002/jbm.a.33276
- K. Sunada
- et al
The external mechanical environment can override the influence of local substrate in determining stem cell fate
J Biomechanics . 2012 Aug 25 ; 45 2483 | DOI : 10.1016/j.jbiomech.2012.07.024
- M. Thorpe
- et al
Hydrostatic pressure acts to stabilize a chondrogenic phenotype in porcine joint tissue derived stem cells
Euro Cells and Mat . 2012 Feb 23 ; 23 121 | DOI : 10.22203/ecm.v023a09
- T. Vinardell
- et al
A comparison of human smooth muscle and mesenchymal stem cells as potential cell sources for tissue-engineered vascular patches
Tissue Eng Part A . 2012 Jan 26 ; 18 986-98 | DOI : 10.1089/ten.TEA.2011.0172
- C. Williams
- et al
Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues
Cryobiol . 2011 Jan 03 ; 62(3) 181-187 | DOI : 10.1016/j.cryobiol.2011.03.001.
- S. Abedini
- et al
Highly bioactive 8 nm hydrothermal TiO2 nanotubes elicit enhanced bone cell response
Adv Eng Mat . 2011 Jan 17 ; 13 B88 | DOI : https://doi.org/10.1002/adem.201080103
- K. Brammer
- et al
Comparative cell behavior on carbon-coated TiO2 nanotube surfaces for osteoblasts vs. osteo-progenitor cells
Acta Biomaterialia . 2011 Jun 01 ; 7 2697 | DOI : https://doi.org/10.1016/j.actbio.2011.02.039
- K. Brammer
- et al
Zirconium oxide nanotube surface prompts increased osteoblast functionality and mineralization
Mat Sci and Eng . 2011 Dec 01 ; 31 1716 | DOI : https://doi.org/10.1016/j.msec.2011.07.016
- C. Frandsen
- et al
Protein composition of microparticles shed from human placenta during placental perfusion: Potential role in angiogenesis and fibrinolysis in preeclampsia
Placenta . 2010 Nov 11 ; 32 63 | DOI : 10.1016/j.placenta.2010.10.011
- S. Guller
- et al
Quantitative proteomic analysis of dexamethasone-induced effects on osteoblast differentiation, proliferation, and apoptosis in MC3T3-E1 cells using SILAC.
Osteo Inter . 2011 Jul 01 ; 22(7) 2175 | DOI : 10.1007/s00198-010-1434-8
- D. Hong
- et al
Affinity-based growth factor delivery using biodegradable, photocrosslinked heparinalginate hydrogels.
J of Cont Rel . 2011 Jul 02 ; 154 258 | DOI : 10.1016/j.jconrel.2011.06.027
- O. Jeon
- et al
The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro.
Biomat . 2011 Jul 23 ; 32 7375 | DOI : 10.1016/j.biomaterials.2011.06.043
- P. Kemoun
- et al
Recognition of β-Strand motifs by RseB is required for E activity in escherichia coli.
J Bacteriol . 2011 Sep 09 ; 193 6179-6186 | DOI : 10.1128/JB.05657-11
- A. Kulp
- et al
Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells.
J Orthop Res. . 2011 Feb 22 ; 29 1075 | DOI : 10.1002/jor.21334
- E. Lau
- et al
Role of mitogen-activated protein kinase in osteoblast differentiation
J Ortho Res . 2010 Aug 30 ; 29 204 | DOI : 10.1002/jor.21222
- F. Lin
- et al
Contribution of bacterial outer membrane vesicles to innate bacterial defense
Biomedcentral . 2011 Dec 01 ; 11 258 | DOI : 10.1186/1471-2180-11-258
- A. Manning
- et al
Polycaprolactone fibres as a potential delivery system for collagen to support bone regeneration
Curr Drug Deliv . 2011 Jul 01 ; 8 448 | DOI : 10.2174/156720111795767951
- S. McNeil
- et al
Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection
Retrovirol . 2011 Jan 12 ; 8(1) 3 | DOI : 10.1186/1742-4690-8-3
- T. Nazari-Shafti
- et al
Schlafen-3 decreases cancer stem cell marker expression and autocrine/juxtacrine signaling in FOLFOX-resistant colon cancer cells
AJP-GI . 2011 May 19 ; 301(2) G347 | DOI : 10.1152/ajpgi.00403
- P-S. Oh
- et al
Effect of various concentrations of antibiotics on osteogenic cell viability and activity
J Orthopaedic Res . 2011 Feb 24 ; 29(7) 1070 | DOI : 10.1002/jor.21343.
- C. Rathbone
- et al
Processing/structure/property relationship of multi-scaled PCL and PCL–HA composite scaffolds prepared via gas foaming and NaCl reverse templating
Biotech and Bioengin . 2010 Dec 14 ; 108 963 | DOI : 10.1002/bit.23018
- A. Salerno
- et al
Cell invasion of poultry-associated Salmonella enterica serovar Enteritidis isolates is associated with pathogenicity, motility and proteins secreted by the type III secretion system.
Microbiol . 2011 Feb 03 ; 157(pt5) 1428 | DOI : 10.1099/mic.0.044461-0.
- D. Shah
- et al
Osteoconductivity and growth factor production by MG63 osteoblastic cells on bioglass-coated orthopedic implants
Biotech and Bioengin . 2011 Feb 01 ; 108 454 | DOI : 10.1002/bit.22955
- F. Tan
- et al
The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells
Biomaterials . 2011 Jun 12 ; 32(31) 7822 | DOI : 10.1016/j.biomaterials.2011.04.034
- J. Wang
- et al
Bioactive collagen membrane as a carrier for sustained release of PDGF
JTisSciEng . 2011 Jan 01 ; 2 4 | DOI : 10.4172/2157-7552.1000110
- S. Yamano
- et al
Localized colonic stem cell transplantation enhances tissue regeneration in murine colitis
JCellMolMed . 2012 Aug 01 ; | DOI : 10.1111/j.1582-4934.2011.01485.x
- Q. Zhou
- et al
Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation
AmJPhysiolGastrointestLiverPhysiol . 2010 May 27 ; 299 G440 | DOI : 10.1152/ajpgi.00098.2010
- C. Barbier de La Serre
- et al
Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides
Biomaterials . 2010 Sep 15 ; 31 8980 | DOI : 10.1016/j.biomaterials.2010.08.027
- B. Choi
- et al
Persistent high glucose concentrations alter the regenerative potential of mesenchymal stem cells
StemCellsDev . 2010 Sep 11 ; 19 1875 | DOI : 10.1089/scd.2010.0009
- C. Cramer
- et al
Requirement of TGFβ signaling for SMO-mediated carcinogenesis
JBiolChem . 2010 Sep 21 ; 285(47) 36570-6 | DOI : 10.1074/jbc.C110.164442
- Q. Fan
- et al
PTHrP 1-141 and 1-86 increase in vitro bone formation
JSurgRes . 2010 Mar 16 ; 162 e9 | DOI : 10.1016/j.jss.2010.02.023
- B. Hildreth
- et al
Morphological and proteomic analysis of early stage of osteoblast differentiation in osteoblastic progenitor cells
ExperimentalRes . 2010 May 17 ; 316 2291 | DOI : 10.1016/j.yexcr.2010.05.011
- D. Hong
- et al
Response of human embryonic stem cells derived mesenchymal stem cells to osteogenic factors and architectures of materials during in vitro osteogenesis
TissueEngineerPartA . 2010 Aug 17 ; 16(11) 3507-14 | DOI : 10.1089/ten.TEA.2010.0097
- J. Hu
- et al
The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres
MolCancer . 2010 Aug 06 ; 6 212 | DOI : 10.1186/1476-4598-9-212.
- S. Kanwar
- et al
Bone morphogenetic protein 6 drives both osteogenesis and chondrogenesis in murine adipose-derived mesenchymal cells depending on culture conditions
BiochemBiophysResCommun . 2010 Sep 09 ; 401(1) 20-5 | DOI : 10.1016/j.bbrc.2010.08.135
- C. Kemmis
- et al
MT1-MMP controls human mesenchymal stem cell trafficking and differentiation
Blood . 2009 Nov 09 ; 115(2) 221-9 | DOI : 10.1182/blood-2009-06-228494
- C. Lu
- et al
Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites.
JRSocInterface . 2009 Jul 29 ; 7(44) 453-65 | DOI : 10.1098/rsif.2009.0255
- S. Misra
- et al
JAK2 V617F uses distinct signalling pathways to induce cell proliferation and neutrophil activation
BritishjournalofHaematology . 2009 Jun 10 ; 150 334 | DOI : https://doi.org/10.1111/j.1365-2141.2010.08249.x
- S. Oku
- et al
Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration
ActaBiomater . 2010 Apr 24 ; 10 4090-4099 | DOI : https://doi.org/10.1016/j.actbio.2010.04.018
- M.G. Raucci
- et al
Characterization of hTERT-immortalized osteoblast cell lines generated from wild-type and connexin43-null mouse calvaria
AmJPhysiolCellPhysiol . 2010 Aug 04 ; 299 C994 | DOI : https://doi.org/10.1152/ajpcell.00544.2009
- M.M. Thi
- et al
The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo.
ActaBiomater . 2010 Apr 18 ; 10 3856 | DOI : 10.1016/j.actbio.2010.04.009
- J. Wang
- et al
A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering
Biomaterials . 2010 Aug 24 ; 31 8684 | DOI : 10.1016/j.biomaterials.2010.07.097
- Z. Zhang
- et al
Opiates inhibit paclitaxel uptake by P-glycoprotein in preparations of human placental inside-out vesicles
BiochemPharm . 2009 Jul 08 ; 78 1272 | DOI : 10.1016/j.bcp.2009.07.002
- S. Hemauer
- et al
Replicative senescence of vascular smooth muscle cells enhances the calcification through initiating the osteoblastic transition
AmJPhysiolHeartCircPhysiol . 2009 Sep 11 ; 297 H1673-H1684 | DOI : 10.1152/ajpheart.00455.2009
- R. Kurimoto-Nakano
- et al
Modular peptides promote human mesenchymal stem cell differentiation on biomaterial surfaces
ActaBiomater . 2009 Aug 06 ; 6 21 | DOI : 10.1016/j.actbio.2009.08.003
- J. Lee
- et al
Inhibitor of DASH proteases affects expression of adhesion molecules in osteoclasts and reduces myeloma growth and bone disease
BritishJofHematology . 2009 Apr 08 ; 145 775 | DOI : 10.1111/j.1365-2141.2009.07696.x
- A. Pennisi
- et al
OTU domain-containing ubiquitin aldehyde-binding protein 1
JBiolChem . 2009 Apr 21 ; 284(24) 16135 | DOI : 10.1074/jbc.M109.007484
- V. Stanisic
- et al
Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells
StemCells . 2009 Jan 01 ; 27 126 | DOI : 10.1634/stemcells.2008-0456
- Z. Zhang
- et al
CD133+CD44+ population efficiently enriches colon cancer initiating cells
AnnSurgOncol . 2008 Jul 29 ; 15 2927 | DOI : 10.1245/s10434-008-0074-0
- N. Haraguchi
- et al
Regulating the age-related oxidative damage, mitochondrial integrity, and antioxidative enzyme activity in fischer 344 rats by supplementation of the antioxidant epigallocatechin-3-gallate
RejuvRes . 2008 Jun 01 ; 11 649 | DOI : 10.1089/rej.2007.0645
- Q. Meng
- et al
Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing
NatureCellBiol . 2008 Mar 02 ; 10 452 | DOI : 10.1038/ncb1708
- P. Scaffidi
- et al
Engineering a disulfide bond to stabilize the calcium binding loop of activated protein C eliminates its anticoagulant but not protective signaling properties
JBiolChem . 2007 Jan 25 ; 282(12) 9251 | DOI : 10.1074/jbc.M610547200
- J. Bae
- et al
Identification of a specific exosite on activated protein C for interaction with protease-activated receptor 1
JBiolChem . 2007 Aug 31 ; 282 25493 | DOI : 10.1074/jbc.M702131200
- L. Yang
- et al