Assay-kits

SensoLyte® 520 Thiol Quantitation Assay Kit “Fluorimetric” - 1 kit

502.00
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  • Cat.Number : AS-72138
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. The SensoLyte® 520 Thiol Quantitation Assay Kit provides a convenient, highly sensitive fluorescent assay for measurement of thiol concentrations. The novel reagent supplied in the kit, upon reaction with thiols, generates fluorescence which can be easily detected at excitation/emission=490/520 nm. The assay has a linear range of 0.04-160 µM thiol.

Specifications

Packaging
Kits components
  • Component A: Thiol Detection Reagent: 5 vials Component B: Reduced Glutathione Standard Stock Solution: 10 mM, 20 µL Component C: Assay Buffer: 50 mL Component D: DMSO: 0.5 mL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Component C can be stored at room temperature for convenience. Protect component A from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.04 uM
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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