SensoLyte® 520 MMP-10 Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-72024
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MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens. This enzyme is involved in several pathological conditions, such as cancer, arthritis and wound healing. SensoLyte® 520 MMP-10 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-10, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Citations
TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin.
Toxicol Appl Pharmacol. . 2014 Feb 24 ; 276(3) 171 | DOI : 10.1016/j.taap.2014.02.010
- D. Abrew
- et al