SensoLyte® 520 HIV Protease Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71147
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The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor 488 (fluorophore) and QXL™ 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte™ Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte™ Fluor 488/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
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Citations
Screening of HIV-1 protease using a combination of an ultra-high-throughput fluorescent-based assay and RapidFire mass spectrometry
J Biomol Screen . 2015 Feb 13 ; 20(5) 606 | DOI : 10.1177/1087057115570838
- J. Meng
- et al
Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease
J BMC . 2010 Mar 01 ; 18(5) 2037 | DOI : https://doi.org/10.1016/j.bmc.2010.01.020
- D. Lu
- et al
Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases
Bioorg Med Chem . 2009 Mar 14 ; 17(8) 3003 | DOI : 10.1016/j.bmc.2009.03.019
- Y. Wei
- et al