Assay-kits

SensoLyte® 520 FAP (Seprase) Assay Kit *Fluorimetric* - 100 Assays (96-well plate)

582.00
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  • Cat.Number : AS-72273
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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The 520 FAP Assay Kit is an assay for screening of enzyme inhibitors or for continuous assay of FAP activity. This kit utilizes a HiLyte Fluor™488/QXL® FRET substrate, that is cleaved by FAP, but not by DPPIV. Upon cleavage by FAP enzyme, the FRET substrate is separated into two fragments resulting in release of HiLyte Fluor™488 fluorescence which can be monitored at excitation/emission = 490 nm/520 nm.


Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Research Area