Tube of Control siRNA duplex pGL2 lucifer (firefly)
Oligos

Control siRNA duplex pGL2 luciferase (firefly) - 5 nmol

€68.50
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  • Cat.Number : SR-CL010-005
  • Availability :
    In production

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In order to monitor your siRNA experiment conditions, Eurogentec provides siRNA control duplexes.
As for any valid experiment, negative and positive controls are necessary.

Control siRNA dup pGL2 lucifer is a positive control available in 5 nmol final quantity. It contains 1 siRNA duplex.
All siRNA control duplexes are IEX-HPLC purified and 100 % MALDI-TOF Mass Spectrometry controlled.
The sequences are validated and published.

RNA interference is licensed under European Patents 1144623, 1214945 and foreign equivalents from Alnylam Pharmaceuticals, Inc., Cambridge, USA and is provided only for use in academic and commercial research (excluding the evaluation or characterization of this product as the potential basis for a siRNA-based drug) and not for any other commercial purposes. Information about licenses for commercial use (including discovery and development of siRNA-based drugs) is available from Alnylam Pharmaceuticals, Inc., 300 Third Street, Cambridge, MA 02142, USA.

Specifications

Packaging
Kits components
  • 1 tube containing 5 nmoles of dried duplex siRNA 1 tube containing 1 mL Rnase-free water
Quantity & Purity
Purification Method
  • IEX-HPLC
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • To make up to 100 µM concentration, add 50.0 µl of recommended buffer. To make up to 20 µM concentration, add 250.0 µl of recommended buffer. To make up to 5 µM concentration, add 1.00 ml of recommended buffer.
Storage Buffer
  • Please use RNase-free solution
Storage Conditions
  • Store siRNAs (and RNA oligos in general) as a dry pellet at –20 °C (or preferably -70 °C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at –70 °C and avoid contact with RNases. siRNA should be resuspended to a convenient stock concentration (e.g. 50 µM), and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, siRNA oligos should be kept dried.
Activity
Biomarker Target
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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