Peptides
Caspase 3 (Apopain) Substrate 1f, fluorogenic - 5 mg
$221.00
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- Cat.Number : AS-25273-5
- Manufacturer Ref. :
-
Availability :
In production
Alternative choices
Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1;
AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.
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Citations
Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites.
Protein Express Purification . 2009 Sep 25 ; 69(1) 29 | DOI : 10.1016/j.pep.2009.09.014
- Y. Lee
- et al