5(6)-FAM [5-(and-6)-Carboxyfluorescein] UltraPure Grade Mixed Isomers
- Cat.Number : AS-81002-1
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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
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Citations
Measurement of flow velocity and inference of liquid viscosity in a microfluidic channel by fluorescence photobleaching.
Langmuir . 2014 Apr 14 ; 30(16) 4868 | DOI : 10.1021/la404891g
- N.J. Carroll
- et al